DNA

Part:BBa_K2184037:Design

Designed by: Margalit Mualem   Group: iGEM16_Danci-K8   (2016-09-30)


mutant SNP for non taster NaCl & Sour taste and guidRNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 104
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 104
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 104
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 104
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Engineered mutant SNP for non taster NaCl & Sour taste by swich G with A base. Engineered guidRNA to this SNP using a tool called Crispr.MIT.Edu. guide was the same for SNP. the SNP made enough of a difference to create or destroy a PAM site. as Cas9 would cut one kind and not the other (PAM is NGG, so if one SNP had NGG and one had NGC as a SNP, We engineered a guide upstream to this PAM and expect Cas9 to cut the NGG form and not the NGC.)


Source

mutant SNP for non taster NaCl & Sour taste- Human genomic

References