DNA
Part:BBa_K2184037:Design
Designed by: Margalit Mualem Group: iGEM16_Danci-K8 (2016-09-30)
mutant SNP for non taster NaCl & Sour taste and guidRNA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 104
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 104
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 104
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 104
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Engineered mutant SNP for non taster NaCl & Sour taste by swich G with A base. Engineered guidRNA to this SNP using a tool called Crispr.MIT.Edu. guide was the same for SNP. the SNP made enough of a difference to create or destroy a PAM site. as Cas9 would cut one kind and not the other (PAM is NGG, so if one SNP had NGG and one had NGC as a SNP, We engineered a guide upstream to this PAM and expect Cas9 to cut the NGG form and not the NGC.)
Source
mutant SNP for non taster NaCl & Sour taste- Human genomic